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7th Indo-US, JNU, Delhi
8th Indo-US, SGPGI, Lucknow
1st Turkish-US Workshop
9th Indo-US, Bangalore
10th Indo-US, Bhubaneswar
1st ASEAN Workshop
2nd Turkish-US Workshop
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Workshop Report
9th Indo-US Flow Cytometry Workshop
"Flow Cytometry in Stem Cell Biology and Regenerative Medicine”
Manipal Institute for Regenerative Medicine, Manipal University
National Centre for Biological Sciences, Tata Institute of Fundamental Research
Bangalore, India
July 19-25, 2008
Photo Album
The main objective of this workshop was to interface faculty and experts in laser flow cytometry from India and abroad with advanced research students interested in the study of stem cells (embryonic and cancer) and tissue regeneration.
Drs. L. Scott Cram, Indresh Kaur, Awtar Krishan, William Telford and Vincent Shankey from the USA, Dr. Vivek Tanavde from Singapore and Dr. Michael Ormerod from the UK supported by local faculty (Drs. H Krishnamurthy, Annapoorni Rangarajan, Satish Totey and Sudhir Krishna) comprised just a part of the teaching team.
On the evening of July 19th, the faculty met and planned our lectures and tutorials. From July 20th to 25th we presented lectures in the morning followed by visits to the National Centre for Biological Research Flow Cytometry labs. In the lab, a team of instructors supported by engineers and technicians provided by the host institution and Beckman Coulter, Becton Dickinson and Guava Technologies demonstrated the various methods used in the cellular analysis of: 1. Cell Cycle and proliferation, 2. Phenotype and markers, 3. Apoptosis, 4. Stem cells, 5. Quality control and data collection and interpretation. Demonstrations illustrated the use of sophisticated methods for the study of stem cells from tumors and hematopoietic tissues. After dinner tutorials were held from 10-11 PM and hosted by one to two faculty members who held open discussions on a variety of subjects. Most of these sessions lasted until mid-night and were attended by most of the students.
A major focus of the lab demonstrations was to teach the attendees the new methods for the study of Side Populations (SP-stem cells) from hematopoietic/embryonic tissues and tumor cell lines and tumors. The students were taught the use of electronic cell volume for the detection of primitive stem cells. Students were provided with protocols, a CD with review articles and reprints of relevant topics and a list of resources available to them. Methods for rapid analysis of cell cycle, DNA aneuploid and proliferation were taught. In Vitrogen, Inc had sent Dr. Vo who lectured on the use of the new rapid proliferation CLICK assay. Dr. Krishan illustrated the use of electronic volume for the study of very small stem cells in hematopoietic tissues. Dr. Vincent Shankey demonstrated the use of flow cytometry for the study of signal transduction and kinases. Beckman Coulter, Becton Dickinson and In Vitrogen provided reagents, dyes and antibodies for the demonstrations.
Out of 63 applicants, 31 were selected for attendance. These students were exposed to lectures that reviewed the topics; lab demonstrations, which illustrated the methods and tutorials, where they had the opportunity to discuss the subject on a one on one basis with the faculty and fellow attendees. We believe this combination of didactic lectures followed by lab demonstrations and open discussions achieved the most in training and refining the analytical skills of the attendees for study of normal and tumor cells. Based on input from the students, we believe that most of the students learned the latest methods for study of tumor and stem cells and should be able to collect data of scientific validity in their own laboratories. They were provided with email contacts so that in case they need any additional help they can contact the faculty members and seek their assistance.
Based on presentations made in this workshop we intend to compile a book for possible publication. Details of the chapters to be included are being worked out.
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