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Dr. Martin Adelmann
Ms. Raquel Cabana
Dr. L. Scott Cram
Dr. Madhu Dikshit
Dr. Sumeet Gujral
Mr. Michael Keeney
Dr. Awtar Krishan
Dr. Mike Ormerod
Dr. Vincent Shankey
Dr. Arvinder Singh
Dr. Ranbir Sobti
Dr. Vivek Tanavde
Dr. William G. Telford
Dr. Rakesh Singal
Mrs. Veena Kapoor
Mr. Ron Hamelik
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 Core Faculty
Ms. Raquel Cabana
Raquel Cabana, received her education from the National University of Cordoba, Argentina. Raquel specializes in Immunology and laboratory diagnostics in Autoimmune, Allergic, Infectious, and Immunodeficiency diseases. Since 2002 Ms Cabana has been Laboratory Manager at NPE Systems, Inc. She is responsible for development of new applications, systems validation and customer support at NPE. Raquel has participated in several flow cytometric studies in the US and abroad focusing on the use of "electronic volume" in conjunction with multicolor fluorescence in biomedical research.
Lecture
The concept of using Electronic Volume in Commercial Flow Cytometry is new, since traditional Flow Cytometers use forward angle light scatter (FSC) to approximate the cell size. The Cell Lab QuantaT Flow Cytometers introduces a new generation of Flow Cytometers that have the ability to measure Electronic Volume based on the Coulter principle (electronic impedance), which is more reliable and accurate for determination of cell/particle volume. The Electronic Volume in conjunction with the other QuantaT parameters: Side Scatter and 3 Fluorescence detectors, provides the researchers an innovative tool for measurement and identification of cells, nuclei or particles in a broad range of 0.7 to 40-microns using an exclusive triangular Flow Cell. Other new concepts are also introduced with this technology: Fluorescent Concentration (for internal dyes: such as propidium iodide or DAPI) and Fluorescence Surface Density (for surface markers as Monoclonal Antibodies labeled with fluorochromes), permitting the estimation of the concentration of the dye, stain or fluorochorome per cell or particle.
Tutorial
Ms. Cabana will focus on demonstration of electronic volume vs. other parameters for the study of apoptosis, proliferation and stem cell analysis.
Relevant Literature
Krishan A, Cabana R. Flow Cytometric Analysis of Electronic Nuclear Volume and DNA content in Normal Mouse Tissues. Cell Cycle 3:3, 380-383, 2004.
Thomas R, Krishan A, Robinson D, Sams C, Costa F. NASA/American Cancer Society High Resolution Flow Cytometry Project-I. Cytometry 43:2-11, 2001.
Krishan A, Wen J, Thomas R, Sridhar K, Smith W. NASA/American Cancer Society High-Resolution Flow Cytometry Project-III. Multiparametric Analysis of DNA Content and Electronic Nuclear Volume in Human Solid Tumors.
Krishan A, Dandekar P, Nathan N, Hamelik R, Miller C, Shaw J. DNA Index, Genome Size and Electronic Nuclear Volume of Vertebrates from the Miami Metro Zoo. Cytometry Par A 65A: 26-34, 2005.
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