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Dr. Martin Adelmann
Ms. Raquel Cabana
Dr. L. Scott Cram
Dr. Madhu Dikshit
Dr. Sumeet Gujral
Mr. Michael Keeney
Dr. Awtar Krishan
Dr. Mike Ormerod
Dr. Vincent Shankey
Dr. Arvinder Singh
Dr. Ranbir Sobti
Dr. Vivek Tanavde
Dr. William G. Telford
Dr. Rakesh Singal
Mrs. Veena Kapoor
Mr. Ron Hamelik
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 Core Faculty
Dr. Scott Cram
Dr. L. Scott Cram received his Ph.D. in Biophysics from the Pennsylvania State University. At Los Alamos National Laboratory he developed a multidisciplinary research program and created the National Flow Cytometry Resource. He was Director of the Life Science Division and Program Director for the Office of Science Programs at Los Alamos. Dr. Cram developed the mammalian chromosome identification and sorting techniques while on a Max-Planck Society fellowship at the Max-Planck-Institut for biophysikalische Chemie in Goettingen. The techniques he developed for chromosome sorting formed the basis on which the human genome National Laboratory Chromosome Specific Gene Library Project was founded. He is a charter member and former president of the International Society for Analytical Cytology.
Lecture
Dr. Cram's first lecture is designed to introduce the concepts, instrumentation, and new flow cytometry capability developments. The material will be introductory and educational for the purpose of giving everyone a common level of understanding - from which more detailed information will be developed during the workshop.
Tutorial
will discuss chromosome sorting for research and clinical applications. The initial stages of the human genome program required the creation of chromosome specific libraries. These phage and cosmid libraries were created using large numbers of flow sorted human chromosomes. From the initial stages of the human genome program to the present time, flow cytogenetics has made numerous contributions to the outcome as well as to closely related genomic and clinical applications. The technology associated with sorting chromosomes will be described from the standpoint of fluorescent probes, measurable characteristics, data interpretation, data display, and the techniques of actual sorting for different applications. Clinical applications will also be described both from the standpoint of opportunities and limitations. The construction of specific FISH probes and the sorting of chromosomes from other
Relevant Literature
G. van den Engh, B. Trask, L. S. Cram, and M. F. Bartholdi, Preparation of Chromosome Suspensions for Flow Cytometry, Cytometry 5: 108 (1984).
L. S. Cram, C.S. Bell, and J.J. Fawcett, Chromosome Sorting and Genomics, Methods in Cell Science, 24: 27-35 (2002).5-
L.S. Cram, J.W. Gray, and N.P. Carter, Cytometry and Genetics, Cytometry, 58: 33-36 (2004).
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