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Dr. Martin Adelmann
Ms. Raquel Cabana
Dr. L. Scott Cram
Dr. Madhu Dikshit
Dr. Sumeet Gujral
Mr. Michael Keeney
Dr. Awtar Krishan
Dr. Mike Ormerod
Dr. Vincent Shankey
Dr. Arvinder Singh
Dr. Ranbir Sobti
Dr. Vivek Tanavde
Dr. William G. Telford
Dr. Rakesh Singal
Mrs. Veena Kapoor
Mr. Ron Hamelik
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 Core Faculty
Mrs. Veena Kapoor
Mrs. Veena Kapoor graduated from the Rajasthan University, India. She joined the American Red Cross, Rockville, MD, USA in 1986, where she was involved in transplantation immunology, tolerance induction and peripheral blood stem cells characterization. She is now Laboratory Manager of the Experimental Transplantation and Immunology Branch Flow Cytometry Lab at the NCI/NIH in Bethesda, MD. She is responsible for management of a multi-instrument basic research core facility serving over 100 NCI investigators. She has working experience in operating a variety of flow cytometry instrumentation (including the FACSVantage SE DiVa cell sorter, FACSAria, BD LSRII, FACScan and FACSCalibur analyzers, Compucyte laser scanning cytometer, NPE analyzer). Her current research interests are in stem cell and telomerase biology.
Lab
Mrs. Kapoor will work with Dr. Telford in presenting a hands-on laboratory aimed at stem and early progenitor cell analysis on the Beckman Coulter/NPE Systems Cell Lab Quanta SC, a bench top flow cytometer specifically modified for stem cell analysis. This system can analyze Hoechst 33342 side population (SP) phenotype stem cells and progenitors, and the laboratory will train participants in murine and/or human cell preparation, analysis and data interpretation. The laboratory will also deal with aldehyde dehydrogenase (ALDH) detection as a phenotype of stem cells and progenitors, using fluorogenic enzyme substrates and the same instrument platform. Extracellular membrane protein markers of stems cells phenotype will also be discussed and possibly demonstrated.
Relevant Literature
Cabana R, Frolova EG, Kapoor V, Thomas RA, Krishan A, Telford WG. The minimal instrumentation requirements for hoechst side population analysis: stem cell analysis on low-cost flow cytometry platforms. Stem Cells. 2006 Nov; 24(11):2573-81.
Telford W, Kapoor V, Jackson J, Burgess W, Buller G, Hawley T, Hawley R. Violet laser diodes in flow cytometry: An update. Cytometry A. 2006 Nov 1; 69(11):1153-60.
Zhang H, Chua KS, Guimond M, Kapoor V, Brown MV, Fleisher TA, Long LM, Bernstein D, Hill BJ, Douek DC, Berzofsky JA, Carter CS, Read EJ, Helman LJ, Mackall CL. Lymphopenia and interleukin-2 therapy alter homeostasis of CD4+CD25+ regulatory T cells. Nat Med. 2005 Nov; 11(11):1238-43.
Kapoor V. and Telford W.G. Telomere length measurement by fluorescence in situ hybridization and flow cytometry. In Flow Cytometry Protocols, Second Edition, (Hawley T.S. and Hawley, R.G., eds.) Methods in Molecular Biology, Vol. 263, 2004, Humana Press, pp. 385-398.
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