Cytometry Workshops

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Core Faculty

Dr. Awtar Krishan

Awtar Krishan obtained PhD in Zoology (cytogenetics) from the Panjab University, Chandigarh, India (1962) and a PhD in Micro-Anatomy from the Univ. of Western Ontario, Canada (1963). From 1965 to 1977, he was at the Sidney Farber Cancer Institute, Harvard Medical School, Boston, USA as Head of the Tissue Ultrastructure and Experimental Pathology Labs. He transferred to the University of Miami-School of Medicine and from 1980 to 1993 was Scientific Director of the Comprehensive Cancer Center. Most of his work has focused on avian cytogenetics, experimental therapeutics and analytical cytology. He developed flow cytometric techniques for rapid analysis of DNA content by the propidium iodide staining method, monitoring of fluorescent drug retention and resistance and for hormone receptor and marker expression in tumor cells. Some of his recent work has focused on monitoring of cell volume and marker expression in human stem cells from apheresis samples and body cavity fluids.

Dr. Krishan has organized cytometry workshops in Egypt, Turkey, Kuala Lumpur, Singapore and Shanghai.. These activities are described in the website: http://www.cytometryworkshops.com. He is Co-Chair of the Education Committee and Chair of the Asia Task Force of the International Society for Advancement of Cytometry (ISAC).

Lab Module

Flow cytometry is a rapid method for detection of stem cells based on marker expression and other biochemical and physiological characteristics. In association with Ron Hamelik, Urmi Chatterji and Shipra Tukhral we will demonstrate use of different assays for the detection of stem cells.

Relevant Literature

Krishan A. Rapid flow cytofluorometry analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 66:188- 193, 1975.

Sharma S, Cabana R, Shariatmadar S, Krishan A. Cellular volume and marker expression in human peripheral blood apheresis stem cells. Cytometry A. 73A:160-167, 2008.

Krishan A, Ganjei-Azar P, Hamelik R, Sharma D, Reis I, Nadji M. Flow immunocytochemistry of marker expression in cells from body cavity fluids. Cytometry A. 77:132-43, 2010. PMID: 19899128

Krishan A, Sharma D, Sharma S, Hamelik RM, Ganjei-Azar P, Nadji M. ALDH(+)/CD44(+)/CD24(-) expression in cells from body cavity fluids. Cytometry B Clin Cytom. 78:176-82, 2010. PMID: 20043317.

Hamelik RM, Krishan A. Click-iT assay with improved DNA distribution histograms. Cytometry A. 75: 862-5. 2009. PMID: 19658154.