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Dr. Martin Adelmann
Ms. Raquel Cabana
Dr. L. Scott Cram
Dr. Madhu Dikshit
Dr. Sumeet Gujral
Mr. Michael Keeney
Dr. Awtar Krishan
Dr. Mike Ormerod
Dr. Vincent Shankey
Dr. Arvinder Singh
Dr. Ranbir Sobti
Dr. Vivek Tanavde
Dr. William G. Telford
Dr. Rakesh Singal
Mrs. Veena Kapoor
Mr. Ron Hamelik
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 Core Faculty
Dr. Awtar Krishan
Dr. Awtar Krishan obtained his PhDs from the Panjab University (1962) and the Univ. of Western Ontario, Canada (1963) in Cytogenetics and Anatomy. From 1965 to 1977, he was at the Sidney Farber Cancer Institute, Harvard Medical School as Head of the Tissue Ultrastructure and Experimental Pathology Labs. He was Scientific Director of the Comprehensive Cancer Center University of Miami-School of Medicine from 1980 to 1993. He has developed flow cytometric techniques for rapid analysis of DNA content by hypotonic propidium iodide, monitoring of drug retention and resistance, and more recently for monitoring hormone receptor expression in archival tumors.
Lecture
This presentation will focus on two recent developments in flow cytometric evaluation of human solid tumors, body cavity fluids and stem cells. The first half of the presentation will be on development and use of a new flow cytometer, capable of simultaneously measuring electronic cell volume and fluorescence. By two-parameter analysis of nuclear volume vs. DNA content of human breast tumors and cells from body cavity fluids, one can identify sub-populations of normal and tumor cells (1). The second part of the presentation will focus on flow cytometric monitoring of drug transport and efflux in drug resistant cells and stem cells (2). Flow cytometric methods for monitoring of receptors for estrogen, progesterone, androgen and vitamin D in formalin fixed-paraffin embedded human breast and prostate tumors will be discussed (3).
Lab
By monitoring intracellular retention of fluorochromes and the effect of various drug efflux blockers, flow cytometry can be used for rapid identification of drug resistant tumor cells, stem cells and of modulators that can reduce resistance by blocking the efflux pump. This wet lab will focus on the proper use of the fluorochromes needed for monitoring of drug resistance and its modulators.
Relevant Literature
Krishan A, Wen J, Thomas RA, Sridhar KS, Smith WI Jr NASA/American Cancer Society high-resolution flow cytometry project - III. Multiparametric analysis of DNA content and electronic nuclear volume in human solid tumors. Cytometry 43: 16-22. 2001.
Krishan A. Monitoring of cellular resistance to cancer chemotherapy: drug retention and efflux. Methods Cell Biol. l64:193-209. 2001.
Krishan A, Arya P, Ganjei-Azar P, Shirley SE, Escoffery CT and Nadji M. Androgen and Vitamin D receptor expression in archival human breast tumors. Clinical Cytometry. 58b: 53-60, 2004.
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