Cytometry Workshops

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Dr. Martin Adelmann
Ms. Raquel Cabana
Dr. L. Scott Cram
Dr. Madhu Dikshit
Dr. Sumeet Gujral
Mr. Michael Keeney
Dr. Awtar Krishan
Dr. Mike Ormerod
Dr. Vincent Shankey
Dr. Arvinder Singh
Dr. Ranbir Sobti
Dr. Vivek Tanavde
Dr. William G. Telford
Dr. Rakesh Singal
Mrs. Veena Kapoor
Mr. Ron Hamelik

 

Core Faculty

Dr. Arvinder Singh

Dr. Arvinder Singh obtained his Ph.D from Institute of Microbial Technology, IMTECH, affiliated to Panjab University, Chandigarh in 1993. His main area of research interest has been infectious diseases immunology, mainly malaria & HIV. He has been involved with the technology of flow cytometry since 1995 and played a significant role in its promotion and popularization in India and south East Asia. He has been a flow cytometer trainer in clinical as well as research applications, mainly multicolor immunophenotyping, cell cycle, stem cell analysis and high speed cell sorting. He is presently working with Dako Denmark A/S as Scientific & Business Adviser for Indian sub-continent region. He has several publications, 2 patents and been an invited speaker in various national and international meetings. Recently he has been nominated as a member co-chair on Membership Services Committee of International Society for Analytical Cytology.

Lecture

Recent updates in high speed cell sorting
Flow Cytometry is the measurement of single cells in a fluid suspension. The major benefit of flow cytometers is the capability to rapidly measure and record multiple characteristics of a large number of cells simultaneously at rates that can exceed 50,000 cells per second, so that even low-frequency cell populations can be quantitated with a high degree of statistical accuracy. Additionally, flow cytometry instrumentation with cell sorting capability provides the ability to recover a selected cell subpopulation in high purity (>99%) from a heterogeneous mixture of cells. The recent developments allow the cells to be sorted at the speed up to 70,000 cells per second with > 99% purity maintaining a very high viability. The subpopulation can then be used in assays to determine biological function or in other molecular studies. Today, flow cytometry provides quantitative and qualitative measurement of heterogeneous cell populations based on antigen expression, enzyme activity, intracellular proteins, gene expression, cell kinetics, DNA content, side population stem cells and other molecular applications.