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Dr. Martin Adelmann
Ms. Raquel Cabana
Dr. L. Scott Cram
Dr. Madhu Dikshit
Dr. Sumeet Gujral
Mr. Michael Keeney
Dr. Awtar Krishan
Dr. Mike Ormerod
Dr. Vincent Shankey
Dr. Arvinder Singh
Dr. Ranbir Sobti
Dr. Vivek Tanavde
Dr. William G. Telford
Dr. Rakesh Singal
Mrs. Veena Kapoor
Mr. Ron Hamelik
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 Core Faculty
Dr. William G. Telford
Dr. William G. Telford obtained his Ph.D. in microbiology from the Michigan State University in 1994 and did his postdoctoral training in immunology at The University of Michigan Medical School. He joined National Cancer Institute, NIH in 1999, and at present is Director of the flow cytometry core laboratory in the NCI Experimental Transplantation and Immunology Branch. Dr. Telford's research interests are in integration of: novel solid-state lasers into flow cytometers, stem cell detection and characterization and apoptosis.
Lecture
Flow cytometry plays a critical role in the identification and characterization of stem cells. In this seminar we will review recent advances in stem cell analysis by flow cytometry, including both traditional and novel cell surface phenotyping approaches; physiological characteristics such as ABC transporter activity using pump-specific efflux probes; and flow cytometric measurement of other stem cell-specific physiological characteristics, including aldehyde dehydrogenase activity. This portion of the talk will be practical in nature, describing both specific laboratory methods and the appropriate instrumentation for detecting these characteristics. The talk will then focus on development of new less expensive flow cytometers for stem cell analysis.
Relevant Literature
Telford WG (2004) Small lasers in flow cytometry (invited book chapter). In Methods in Molecular Biology Volume 263, Flow Cytometry Protocols, 2nd Edition, T.S. Hawley and R.G. Hawley, eds., Humana Press, London, UK., pp. 399-418.
Telford WG, Frolova EG (2004) Discrimination of Hoechst side population in mouse bone marrow with violet and near-UV laser diodes. Cytometry 57A, 45-52.
Terunumi A, Jackson KL, Kapoor V, Telford WG, Vogel JC (2003) Side population keratinocytes resembling bone marrow side population stem cells are distinct from label-retaining keratinocyte stem cells. Journal of Investigative Dermatology 121, 1095-1103.
Hawley TS, Telford WG, Ramezani A, Hawley RG (2001) "Rainbow" reporters for multispectral marking and lineage analysis of hematopoietic stem cells (review article). Stem Cell 19, 118-124.
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